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anti mouse cd8 α  (Bio X Cell)


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    Structured Review

    Bio X Cell anti mouse cd8 α
    Anti Mouse Cd8 α, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd8+antibodies/InVivoMAb+anti-mouse+CD8%CE%B2/pm42829916-411-4-19
    Average 96 stars, based on 205 article reviews
    anti mouse cd8 α - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Control:

    Article Title: Liver-directed AAV-IL-10 therapy enhances CD8 + T cell-mediated immunity against hepatocellular carcinoma.
    Article Snippet: .. In the CD8+ T- cell depletion experiment, mice received anti- CD8 antibodies (Bio X Cell, clone 2.43) or isotype control antibodies (Bio X Cell, clone LTF- 2) starting 1 day before AAV administration and continuing until the end of the experiment (100 μg/mouse, two times a week). ..

    Article Title: Liver-directed AAV-IL-10 therapy enhances CD8+ T cell-mediated immunity against hepatocellular carcinoma
    Article Snippet: .. In the CD8 + T-cell depletion experiment, mice received anti-CD8 antibodies (Bio X Cell, clone 2.43) or isotype control antibodies (Bio X Cell, clone LTF-2) starting 1 day before AAV administration and continuing until the end of the experiment (100 μg/mouse, two times a week). ..

    Bioprocessing:

    Article Title: Liver-directed AAV-IL-10 therapy enhances CD8 + T cell-mediated immunity against hepatocellular carcinoma.
    Article Snippet: .. In the CD8+ T- cell depletion experiment, mice received anti- CD8 antibodies (Bio X Cell, clone 2.43) or isotype control antibodies (Bio X Cell, clone LTF- 2) starting 1 day before AAV administration and continuing until the end of the experiment (100 μg/mouse, two times a week). ..

    Article Title: Liver-directed AAV-IL-10 therapy enhances CD8+ T cell-mediated immunity against hepatocellular carcinoma
    Article Snippet: .. In the CD8 + T-cell depletion experiment, mice received anti-CD8 antibodies (Bio X Cell, clone 2.43) or isotype control antibodies (Bio X Cell, clone LTF-2) starting 1 day before AAV administration and continuing until the end of the experiment (100 μg/mouse, two times a week). ..

    Injection:

    Article Title: Intrinsic RB activation induces tumoral and stromal anti-tumor responses that limit triple-negative breast cancer.
    Article Snippet: .. For the first depletion, 0.5mg of anti-CD8 antibodies (BioXCell, BE0004-1) were injected intraperitoneally one day before Dox treatment per mouse. ..

    Article Title: Irradiated Tumor Cell‐Derived Microparticles Activate Systemic Anti‐Tumor Immunity via the STING/NLRP3/GSDMD Axis in Neutrophils
    Article Snippet: In the neutrophil depletion study, mouse anti‐Ly6G antibodies (#1A8; BioXCell) were diluted in PBS and administered via intraperitoneal injection (q3d ×3 doses, the first dose at 400 μg/mouse and the next two doses at 100 μg/mouse). .. In the T cell depletion study, mouse anti‐CD8 antibodies (BE0061; BioXCell) or mouse anti‐CD4 antibodies (BE0003‐1; BioXCell) were diluted in PBS and administered via intraperitoneal injection (q3d ×3 doses, the first dose at 200 mg/mouse and the next two doses at 100 mg/mouse). .. In the macrophage depletion study, clodronate liposomes (F70101CAC; FormuMax) were intraperitoneally injected (q3d ×3 doses, the first dose at 200 μL/mouse and the next two doses at 150 μL/mouse).

    Article Title: Intrinsic RB activation induces tumoral and stromal anti-tumor responses that limit triple-negative breast cancer
    Article Snippet: .. For the first depletion, 0.5 mg of anti-CD8 antibodies (BioXCell, BE0004-1) were injected intraperitoneally one day before Dox treatment per mouse. ..

    Liposomes:

    Article Title: Commensal enteric virome regulates intestinal carbohydrate digestion and absorption.
    Article Snippet: A Mouse IL-22 High Sensitivity ELISA Kit (EK222HS, Multi sciences), IL-23 ELISA Kit (EK223, Multi sciences), insulin (MM-0579M2, MEIMIAN), IL-6 (R-S-M0007, RUISAIQI), IL-1β (R-S-M0003, RUISAIQI), TNF-α (R-S-M0002, RUISAIQI) and adiponectin (R-S-M10108, RUISAIQI) were utilized to measure the protein concentrations according to the manufacturer’s instructions. .. T cells were depleted using 250 μg of anti-CD4, anti-CD8 antibodies (BE0003-41, BE0004-1, BioXcell), while F4/80+ APCs were eliminated with clodronate liposomes (40337ES08, Yeasen) following the manufacturer’s instructions. .. For cytokine neutralization, 100 μg of anti-IL-22 (16-7222-82, Thermo Fisher) or anti-IL-23 (p19) (BE0313, BioXcell) was administered.

    other:

    Article Title: Combination of PARP and KRAS G12D inhibitors enhances therapeutic efficacy by exploiting vulnerabilities in PDAC
    Article Snippet: The proportion of EdU-positive cells was quantified by counting at least 100 cells per field from at least five random fields per sample, using ImageJ software.



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    Overexpression of UPF1 in ccRCC inhibits M2 macrophage polarization and enhances the tumor suppressor function of <t>CD8</t> + T cells, Caki-1 cell groups of sh-NC+PBS, sh-UPF1+PBS, or sh-UPF1 + 2-DG, and the conditioned medium was used to culture and polarize M0 macrophages. The polarized macrophages were cultured with activated CD8 + T cells. (A) CFSE assessed CD8 + T cell proliferative capacity. (B) Flow cytometry measured apoptosis rate of CD8 + T cells in different co-culture systems. (C) CD8 + T cells from culture systems were co-cultured with normal Caki-1 cells, with LDH assays determining cytotoxic effect of CD8 + T cells. *, p < 0.05. cRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1; 2-DG, 2-Deoxy-D-glucose; CFSE, Carboxyfluorescein succinimidyl ester.
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    Cytek Biosciences pe anti human cd8 antibody tonbo 50 0088 t100
    Overexpression of UPF1 in ccRCC inhibits M2 macrophage polarization and enhances the tumor suppressor function of <t>CD8</t> + T cells, Caki-1 cell groups of sh-NC+PBS, sh-UPF1+PBS, or sh-UPF1 + 2-DG, and the conditioned medium was used to culture and polarize M0 macrophages. The polarized macrophages were cultured with activated CD8 + T cells. (A) CFSE assessed CD8 + T cell proliferative capacity. (B) Flow cytometry measured apoptosis rate of CD8 + T cells in different co-culture systems. (C) CD8 + T cells from culture systems were co-cultured with normal Caki-1 cells, with LDH assays determining cytotoxic effect of CD8 + T cells. *, p < 0.05. cRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1; 2-DG, 2-Deoxy-D-glucose; CFSE, Carboxyfluorescein succinimidyl ester.
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    Bio X Cell anti cd8 antibody
    Overexpression of UPF1 in ccRCC inhibits M2 macrophage polarization and enhances the tumor suppressor function of <t>CD8</t> + T cells, Caki-1 cell groups of sh-NC+PBS, sh-UPF1+PBS, or sh-UPF1 + 2-DG, and the conditioned medium was used to culture and polarize M0 macrophages. The polarized macrophages were cultured with activated CD8 + T cells. (A) CFSE assessed CD8 + T cell proliferative capacity. (B) Flow cytometry measured apoptosis rate of CD8 + T cells in different co-culture systems. (C) CD8 + T cells from culture systems were co-cultured with normal Caki-1 cells, with LDH assays determining cytotoxic effect of CD8 + T cells. *, p < 0.05. cRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1; 2-DG, 2-Deoxy-D-glucose; CFSE, Carboxyfluorescein succinimidyl ester.
    Anti Cd8 Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Overexpression of UPF1 in ccRCC inhibits M2 macrophage polarization and enhances the tumor suppressor function of CD8 + T cells, Caki-1 cell groups of sh-NC+PBS, sh-UPF1+PBS, or sh-UPF1 + 2-DG, and the conditioned medium was used to culture and polarize M0 macrophages. The polarized macrophages were cultured with activated CD8 + T cells. (A) CFSE assessed CD8 + T cell proliferative capacity. (B) Flow cytometry measured apoptosis rate of CD8 + T cells in different co-culture systems. (C) CD8 + T cells from culture systems were co-cultured with normal Caki-1 cells, with LDH assays determining cytotoxic effect of CD8 + T cells. *, p < 0.05. cRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1; 2-DG, 2-Deoxy-D-glucose; CFSE, Carboxyfluorescein succinimidyl ester.

    Journal: Frontiers in Immunology

    Article Title: UPF1-dependent glycolysis driven by LINC00887 facilitates macrophage M2 polarization in clear cell renal cell carcinoma

    doi: 10.3389/fimmu.2026.1833383

    Figure Lengend Snippet: Overexpression of UPF1 in ccRCC inhibits M2 macrophage polarization and enhances the tumor suppressor function of CD8 + T cells, Caki-1 cell groups of sh-NC+PBS, sh-UPF1+PBS, or sh-UPF1 + 2-DG, and the conditioned medium was used to culture and polarize M0 macrophages. The polarized macrophages were cultured with activated CD8 + T cells. (A) CFSE assessed CD8 + T cell proliferative capacity. (B) Flow cytometry measured apoptosis rate of CD8 + T cells in different co-culture systems. (C) CD8 + T cells from culture systems were co-cultured with normal Caki-1 cells, with LDH assays determining cytotoxic effect of CD8 + T cells. *, p < 0.05. cRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1; 2-DG, 2-Deoxy-D-glucose; CFSE, Carboxyfluorescein succinimidyl ester.

    Article Snippet: Antibodies included: FITC Mouse monoclonal to CD68 (ab134351, abcam, UK), Pacific Blue anti-human CD86 Antibody (305417, yajimall, China), CD206 (MMR) Monoclonal Antibody (19.2)-PE (12-2069-42, ThermoFisher, USA), PerCP/Cyanine5.5 Anti-Mouse F4/80 Antibody (E-AB-F0995J, Elabscience, China), PE Anti-CD206 mouse antibody (E-AB-F1135D, Elabscience, China), APC Anti-mouse CD3 antibody (E-AB-F1013E, Elabscience, China), PE Anti-mouse CD8 alpha antibody (E-AB-F1104D, Elabscience, China).

    Techniques: Over Expression, Cell Culture, Flow Cytometry, Co-culture Assay

    LINC00887/UPF1-mediated aerobic glycolysis leads to lactic acid accumulation and facilitates immune evasion in ccRCC in vivo , A syngeneic tumor model was developed by injecting BALB/c mice with Renca cells expressing sh-NC or sh-LINC00887, followed by treatment with sh-NC or sh-UPF1 lentivirus. A-C: Assessment of tumor size (A) , volume (B) , and weight (C) . (D, E) qRT-PCR analyses for LINC00887 (D) and UPF1 (E) mRNA levels. (F) IHC analysis for UPF1, LDH-A, KI67 protein levels. (G) Kit assay for lactate levels in tumors. (H) Proportion of M2 macrophages (F4/80+CD206+) by flow cytometry. (I) Immunofluorescence assay for CD206+ macrophages. (J) Proportion of CD8 + T cells (CD3 + CD8 + ) in tumor tissues. ns, no significant difference. * p < 0.05. cRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1; LDH-A, Lactate dehydrogenase A; CD206, Mannose receptor C-type 1; CD3, Cluster of differentiation 3; CD8, Cluster of differentiation 8.

    Journal: Frontiers in Immunology

    Article Title: UPF1-dependent glycolysis driven by LINC00887 facilitates macrophage M2 polarization in clear cell renal cell carcinoma

    doi: 10.3389/fimmu.2026.1833383

    Figure Lengend Snippet: LINC00887/UPF1-mediated aerobic glycolysis leads to lactic acid accumulation and facilitates immune evasion in ccRCC in vivo , A syngeneic tumor model was developed by injecting BALB/c mice with Renca cells expressing sh-NC or sh-LINC00887, followed by treatment with sh-NC or sh-UPF1 lentivirus. A-C: Assessment of tumor size (A) , volume (B) , and weight (C) . (D, E) qRT-PCR analyses for LINC00887 (D) and UPF1 (E) mRNA levels. (F) IHC analysis for UPF1, LDH-A, KI67 protein levels. (G) Kit assay for lactate levels in tumors. (H) Proportion of M2 macrophages (F4/80+CD206+) by flow cytometry. (I) Immunofluorescence assay for CD206+ macrophages. (J) Proportion of CD8 + T cells (CD3 + CD8 + ) in tumor tissues. ns, no significant difference. * p < 0.05. cRCC, Clear cell renal cell carcinoma; UPF1, Up-frameshift protein 1; LDH-A, Lactate dehydrogenase A; CD206, Mannose receptor C-type 1; CD3, Cluster of differentiation 3; CD8, Cluster of differentiation 8.

    Article Snippet: Antibodies included: FITC Mouse monoclonal to CD68 (ab134351, abcam, UK), Pacific Blue anti-human CD86 Antibody (305417, yajimall, China), CD206 (MMR) Monoclonal Antibody (19.2)-PE (12-2069-42, ThermoFisher, USA), PerCP/Cyanine5.5 Anti-Mouse F4/80 Antibody (E-AB-F0995J, Elabscience, China), PE Anti-CD206 mouse antibody (E-AB-F1135D, Elabscience, China), APC Anti-mouse CD3 antibody (E-AB-F1013E, Elabscience, China), PE Anti-mouse CD8 alpha antibody (E-AB-F1104D, Elabscience, China).

    Techniques: In Vivo, Expressing, Quantitative RT-PCR, Analysis, Flow Cytometry, Immunofluorescence